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We provide a fast and reliable scale-up monoclonal antibody from transient production. Utilizing a dual high-copy vector system, F(ab)2 and Fc fragments are co-transfected into a mammalian cell line. These transfected cells are then amplified in optimized serum-free, suspension culture, and the expressed antibodies are purified with affinity chromatography. Final products are quality-tested with IgG antibody SDS-PAGE Coomassie Blue staining and ELISA. Transient antibody production has the ability to produce the desired quantity of up to 100mg within 2-3 weeks.
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| Transient Antibody Production Workflow
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- Plasmid Vector :
pAB03(with Fc) + Fd
pAB04 + LC
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- Host Cell :
293H
293F
293T
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| Advantages |
Applications |
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- High Affinity Antibody
- Diverse Antibody Repertoire
- No Immunologic Tolerance
- Scalable Antibody Production
- More Stable Antibody Clone
- Antibody Engineering
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- High Affinity Antibody
- Rabbit Monoclonal Antibody
- Modification-State Antibody
- Anti-Idiotype Antibody
- Antibody Pairing and ELISA Kit
- Research and In Vitro Diagnostic
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| Example |
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| Antibody Concentration versus Yield |
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Production Site |
Host Cell |
Antibody Concentration (μg/uL) |
Yield
(Mab μg/Culture Sup mL) |
| Humanized CD3 monoclonal antibody |
Other Company |
293F |
1.08 |
10 |
| Abnova |
293H |
4.84 |
70 |
| 293F |
1.52 |
30 |
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| Flow Cytometry |
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Flow cytometry of human CD3 T cells stained humanized CD3 monoclonal Ab (1 µg/5*105 cells) and counterstained with PE anti-human IgG Ab (1 µg/5*105 cells).
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For any inquiry, please contact : OEM@abnova.com
