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Last updated: 2023/5/29
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TPI1 polyclonal antibody 

  • Catalog # : PAB6740
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of TPI1.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human TPI1.
  • Sequence:
  • C-LKPEFVDIINAKQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 26.7
  • Reactivity:
  • Dog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Immunohistochemistry (2-5 ug/mL)
    Flow Cytometry (10 ug/mL)
    Western Blot (0.001-0.01 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • TPI1 polyclonal antibody (Cat # PAB6740) staining (0.001?g/ml) HepG2 (1), HEK293 (2), HeLa (3) and Jurkat (4) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • TPI1 polyclonal antibody (Cat # PAB6740) (5 ug/mL) staining of paraffin embedded Human Pancreas.Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • TPI1 polyclonal antibody (Cat # PAB6740) (2 ug/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • TPI1 polyclonal antibody (Cat # PAB6740) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 7167
  • Protein Accession#:
  • NP_000356.1;NP_001152759.1
  • Gene Name:
  • TPI1
  • Gene Alias:
  • MGC88108,TPI
  • Gene Description:
  • triosephosphate isomerase 1
  • Gene Summary:
  • This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • -
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