NUP62 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NUP62.
Immunogen
A synthetic peptide corresponding to human NUP62.
Sequence
C-RKEQERSFRITFD
Host
Goat
Theoretical MW (kDa)
53.3
Specificity
All four variants represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NUP62 polyclonal antibody (Cat # PAB6546) (1 ug/mL) staining of nuclear lysates of cell line HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUP62
Entrez GeneID
23636Protein Accession#
NP_0.36478.2;NP_057637.2;NP_714940.1;NP_714941.1Gene Name
NUP62
Gene Alias
DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, SNDI, p62
Gene Description
nucleoporin 62kDa
Gene Ontology
HyperlinkGene Summary
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq
Other Designations
nuclear pore glycoprotein p62
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Interactome
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Publication Reference
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Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.
Carmo-Fonseca M, Kern H, Hurt EC.
Eur J Cell Biol 1991 Jun; 55(1):17.
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Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.
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