FOS (phospho T232) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated COS7 cells, Lane 2: COS7 cells with FOS (phospho T232) polyclonal antibody (Cat # PAB29224) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast carcinoma tissue by FOS (phospho T232) polyclonal antibody (Cat # PAB29224) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human FOS.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T232 of human FOS.
Host
Rabbit
Theoretical MW (kDa)
48
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated COS7 cells, Lane 2: COS7 cells with FOS (phospho T232) polyclonal antibody (Cat # PAB29224) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast carcinoma tissue by FOS (phospho T232) polyclonal antibody (Cat # PAB29224) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — FOS
Entrez GeneID
2353Protein Accession#
P01100Gene Name
FOS
Gene Alias
AP-1, C-FOS
Gene Description
v-fos FBJ murine osteosarcoma viral oncogene homolog
Omim ID
164810Gene Ontology
HyperlinkGene Summary
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq
Other Designations
FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|activator protein 1|cellular oncogene c-fos
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Interactome
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Pathway
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Disease
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