FOS recombinant monoclonal antibody, clone 14C10
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: MCF-7 whole cell lysate, Lane 4: Jurkat whole cell lysate, Lane 5: A549 whole cell lysate, Lane 6: 293 whole cell lysate and Lane 7: 3T3 whole cell lysate with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376).
Immunohistochemistry
Immunohistochemical staining of human adrenal gland tissue with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:81).
Immunohistochemistry
Immunohistochemical staining of human cervical cancer with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:81).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:27). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FOS.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human FOS.
Theoretical MW (kDa)
Calculated MW: 41, 2
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: MCF-7 whole cell lysate, Lane 4: Jurkat whole cell lysate, Lane 5: A549 whole cell lysate, Lane 6: 293 whole cell lysate and Lane 7: 3T3 whole cell lysate with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376).Immunohistochemistry
Immunohistochemical staining of human adrenal gland tissue with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:81).Immunohistochemistry
Immunohistochemical staining of human cervical cancer with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:81).Immunofluorescence
Immunofluorescent staining of HepG2 cells with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:27). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with FOS recombinant monoclonal antibody, clone 14C10 (Cat # RAB04376) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — FOS
Entrez GeneID
2353Protein Accession#
P01100Gene Name
FOS
Gene Alias
AP-1, C-FOS
Gene Description
v-fos FBJ murine osteosarcoma viral oncogene homolog
Omim ID
164810Gene Ontology
HyperlinkGene Summary
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq
Other Designations
FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|activator protein 1|cellular oncogene c-fos
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Interactome
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Pathway
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Disease
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