RORC polyclonal antibody
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More Files
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of RORC.
Immunogen
A synthetic peptide corresponding to amino acids 200-212 at internal region of human RORC.
Sequence
CHLEYSPERGKAE
Host
Goat
Theoretical MW (kDa)
55.8
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_005051.2; NP_001001523.1).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:16000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Western Blot (0.01-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RORC polyclonal antibody (Cat # PAB19661) (0.1 ug/mL) staining of Human Colon (A), (0.03 ug/mL) Liver (B) and (0.01 ug/mL) Testes (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunofluorescence
RORC polyclonal antibody (Cat # PAB19661) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear, cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
RORC polyclonal antibody (Cat # PAB19661) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — RORC
Entrez GeneID
6097Protein Accession#
NP_005051.2;NP_001001523.1Gene Name
RORC
Gene Alias
MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR
Gene Description
RAR-related orphan receptor C
Omim ID
602943Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000015253|RAR-related orphan receptor C, isoform a|RAR-related orphan receptor gamma|nuclear receptor ROR-gamma|retinoic acid-binding receptor gamma|retinoid-related orphan receptor gamma
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Interactomes
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Diseases
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