RB1 (phospho S249) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RB1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S249 of human RB1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RB1 (phospho S249) polyclonal antibody (Cat # PAB0595) in SK-BR-3 cell lysate (35 ug/lane). RB1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RB1 (phospho S249) polyclonal antibody (Cat # PAB0595) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — RB1
Entrez GeneID
5925Protein Accession#
NP_000312;P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma.
Roesch A, Becker B, Meyer S, Hafner C, Wild PJ, Landthaler M, Vogt T.
Modern Pathology: an Official Journal of the United States and Canadian Academy of Pathology, Inc 2005 Apr; 18(4):565.
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Retinoblastoma protein is required for efficient colorectal carcinoma cell apoptosis by histone deacetylase inhibitors in the absence of p21Waf.
Wagner S, Roemer K.
Biochemical Pharmacology 2005 Apr; 69(7):1059.
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Loss of Rb-E2F repression results in caspase-8-mediated apoptosis through inactivation of focal adhesion kinase.
Lieman JH, Worley LA, Harbour JW.
The Journal of Biological Chemistry 2005 Jan; 280(11):10484.
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Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma.
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