RB1 (phospho Thr252) recombinant monoclonal antibody, clone R03-8H1
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HL-60, Lane 2: U2OS and Lane 3: C6 lysates with RB1 (phospho Thr252) recombinant monoclonal antibody, clone R03-8H1 (Cat #RAB01777).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr252 of human RB1.
Theoretical MW (kDa)
Calculated MW: 106 k
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1/50-1/100)
Western Blot (1/500-1/1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HL-60, Lane 2: U2OS and Lane 3: C6 lysates with RB1 (phospho Thr252) recombinant monoclonal antibody, clone R03-8H1 (Cat #RAB01777).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — RB1
Entrez GeneID
5925Protein Accession#
P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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