JUN (phospho S63) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of JUN.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S63 of human JUN.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Dot Blot (1:500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of JUN (phospho S63) polyclonal antibody (Cat # PAB0444) in mouse brain tissue lysate (35 ug/lane). Mouse Phospho-JUN-pS63 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with JUN (phospho S63) polyclonal antibody (Cat # PAB0444) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Dot Blot (Peptide)
Dot blot analysis of JUN (phospho S63) polyclonal antibody (Cat # PAB0444) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — JUN
Entrez GeneID
3725Protein Accession#
NP_002219;P05412Gene Name
JUN
Gene Alias
AP-1, AP1, c-Jun
Gene Description
jun oncogene
Omim ID
165160Gene Ontology
HyperlinkGene Summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
Other Designations
Jun activation domain binding protein|OTTHUMP00000010036|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases.
Cheng J, Perkins ND, Yeh ET.
The Journal of Biological Chemistry 2005 Apr; 280(15):14492.
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Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts.
Quan T, He T, Voorhees JJ, Fisher GJ.
The Journal of Biological Chemistry 2005 Mar; 280(9):8079.
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Identification of endogenous glucocorticoid repressed genes differentially regulated by a glucocorticoid receptor mutant able to separate between nuclear factor-kappaB and activator protein-1 repression.
Bladh LG, Liden J, Dahlman-Wright K, Reimers M, Nilsson S, Okret S.
Molecular Pharmacology 2005 Mar; 67(3):815.
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Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases.
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