JUN (phospho S63) recombinant monoclonal antibody, clone 4A11
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate (not treated), Lane 2: Hela whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 3: A549 whole cell lysate (not treated) and Lane 4: A549 whole cell lysate (treated with Calyculin A 100nM/60 mins) with JUN (phospho S63) recombinant monoclonal antibody, clone 4A11 (Cat # RAB04278).
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with JUN (phospho S63) recombinant monoclonal antibody, clone 4A11 (Cat # RAB04278) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of A549 cells with JUN (phospho S63) recombinant monoclonal antibody, clone 4A11 (Cat # RAB04278) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human JUN.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S63 of human JUN.
Theoretical MW (kDa)
Calculated MW: 48 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate (not treated), Lane 2: Hela whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 3: A549 whole cell lysate (not treated) and Lane 4: A549 whole cell lysate (treated with Calyculin A 100nM/60 mins) with JUN (phospho S63) recombinant monoclonal antibody, clone 4A11 (Cat # RAB04278).Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with JUN (phospho S63) recombinant monoclonal antibody, clone 4A11 (Cat # RAB04278) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of A549 cells with JUN (phospho S63) recombinant monoclonal antibody, clone 4A11 (Cat # RAB04278) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — JUN
Entrez GeneID
3725Protein Accession#
P05412Gene Name
JUN
Gene Alias
AP-1, AP1, c-Jun
Gene Description
jun oncogene
Omim ID
165160Gene Ontology
HyperlinkGene Summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
Other Designations
Jun activation domain binding protein|OTTHUMP00000010036|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
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Interactome
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Pathway
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Disease
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