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Last updated: 2023/3/26

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PKCG (phospho T514) monoclonal antibody, clone PF4 

  • Catalog # : MAB23561
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human PKC gamma.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding T514 of human PKC gamma.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Flow Cytometry
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of C6 cell lysate with PKCG (phospho T514) monoclonal antibody, clone PF4 (Cat # MAB23561). Lane 1: untreated or Lane 2: treated with staurosporine.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of NIH3T3 cell lysate with PKCG (phospho T514) monoclonal antibody, clone PF4 (Cat # MAB23561). Lane 1: untreated or Lane 2: treated with TPA.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells with PKCG (phospho T514) monoclonal antibody, clone PF4 (Cat # MAB23561). Treated with K252a and stained with the secondary antibody only negative control (blue) or treated with K252a (red) or untreated (green).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5582
  • Gene Name:
  • PRKCG
  • Gene Alias:
  • MGC57564,PKC-gamma,PKCC,PKCG,SCA14
  • Gene Description:
  • protein kinase C, gamma
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq
  • Other Designations:
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