PRKCG (phospho Thr514) recombinant monoclonal antibody, clone R02-4F3
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRKCG.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr514 of human PRKCG.
Theoretical MW (kDa)
Calculated MW: 78 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: C6 and Lane 2: Hela lysates with PRKCG (phospho Thr514) recombinant monoclonal antibody, clone R02-4F3 (Cat # RAB01997).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human brain with PRKCG (phospho Thr514) recombinant monoclonal antibody, clone R02-4F3 (Cat # RAB01997).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — PRKCG
Entrez GeneID
5582Protein Accession#
P05129Gene Name
PRKCG
Gene Alias
MGC57564, PKC-gamma, PKCC, PKCG, SCA14
Gene Description
protein kinase C, gamma
Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq
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