E2F1 monoclonal antibody, clone IGD-5
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa and Lane 2: NIH/3T3 cell lysates with E2F1 monoclonal antibody, clone IGD-5 (Cat # MAB19998).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human E2F1.
Immunogen
A synthetic peptide corresponding to human E2F1.
Host
Rabbit
Theoretical MW (kDa)
46.92
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: HeLa and Lane 2: NIH/3T3 cell lysates with E2F1 monoclonal antibody, clone IGD-5 (Cat # MAB19998).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — E2F1
Entrez GeneID
1869Protein Accession#
Q01094Gene Name
E2F1
Gene Alias
E2F-1, RBAP1, RBBP3, RBP3
Gene Description
E2F transcription factor 1
Omim ID
189971Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq
Other Designations
OTTHUMP00000030661|retinoblastoma-associated protein 1
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Interactome
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Pathway
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Disease
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