E2F1 recombinant monoclonal antibody, clone 1D12
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Jurkat whole cell lysate; Lane 2: PC-3 whole cell lysate; Lane3: Rat Heart tissue.
Immunohistochemistry
Immunohistochemistry image of E2F1 recombinant monoclonal antibody, clone 1D12 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with E2F1 recombinant monoclonal antibody, clone 1D12 at 1:50, counter-stained with DAPI.
Flow Cytometry
Overlay histogram showing Hela cells stained with E2F1 recombinant monoclonal antibody, clone 1D12 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and rat E2F1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human E2F1.
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Jurkat whole cell lysate; Lane 2: PC-3 whole cell lysate; Lane3: Rat Heart tissue.Immunohistochemistry
Immunohistochemistry image of E2F1 recombinant monoclonal antibody, clone 1D12 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with E2F1 recombinant monoclonal antibody, clone 1D12 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Hela cells stained with E2F1 recombinant monoclonal antibody, clone 1D12 (red line) at 1:50. -
Gene Info — E2F1
Entrez GeneID
1869Protein Accession#
Q01094Gene Name
E2F1
Gene Alias
E2F-1, RBAP1, RBBP3, RBP3
Gene Description
E2F transcription factor 1
Omim ID
189971Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq
Other Designations
OTTHUMP00000030661|retinoblastoma-associated protein 1
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Interactome
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