mutaFISH™ EGFRwt RNA Probes
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Product Description
mutaFISH™ EGFRwt RNA Probes is designed to detect human EGFR gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT EGFR Primer
2. mutaFISH™ EGFRwt RNA Probe
3. Detection Probe-FITCComparison
Fluorophore
FITC (Excitation Peak (nm): 495; Emission Peak (nm): 519)
Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in PC-9 cells. EGFR gene was detected via green signal (FITC).
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