mutaFISH™ KRAS G12wt G13wt RNA Probes
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More Files
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mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human HCT116 cells. KRAS G12wt and G13wt were detected via red signal (Cy5).
Fig.2 mutaFISH™ staining was performed in situ in human WIDR cells. KRAS G12wt and G13wt were detected via red signal (Cy5).
Fig.3 mutaFISH™ staining was performed in situ in human SW480 cells. KRAS G12wt and G13wt were not detected.
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Specification
Product Description
mutaFISH™ KRAS G12wt G13wt RNA Probes is designed to detect human KRAS G12/G13 wildtype on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT KRAS G12/G13 Primer
2. mutaFISH™ KRAS G12wt RNA Probe 1
3. mutaFISH™ KRAS G12wt RNA Probe 2
4. mutaFISH™ KRAS G13wt RNA Probe
5. Detection Probe-Texas Red XComparison
Fluorophore
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)
Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human HCT116 cells. KRAS G12wt and G13wt were detected via red signal (Cy5).
Fig.2 mutaFISH™ staining was performed in situ in human WIDR cells. KRAS G12wt and G13wt were detected via red signal (Cy5).
Fig.3 mutaFISH™ staining was performed in situ in human SW480 cells. KRAS G12wt and G13wt were not detected. -
Gene Info — KRAS
Entrez GeneID
3845Gene Name
KRAS
Gene Alias
C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2
Gene Description
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Ontology
HyperlinkGene Summary
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq
Other Designations
K-ras p21 protein|Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog|PR310 c-K-ras oncogene|c-K-ras2 protein|c-Kirsten-ras protein|cellular c-Ki-ras2 proto-oncogene|oncogene KRAS2|transforming protein p21|v-Ki-ras2 Kirsten rat sarcoma 2 viral oncoge
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Interactome
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Pathway
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Disease
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