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Last updated: 2015/8/30
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ACTB/ACTC1 polyclonal antibody

  • Catalog # : PAB3161
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ACTB/ACTC1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human ACTB/ACTC1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of ACTB/ACTC1 polyclonal antibody (Cat # PAB3161) in HL-60 cell line lysates (35 ug/lane). ACTB/ACTC1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of ACTB/ACTC (arrow) using rabbit ACTB/ACTC1 polyclonal antibody (Cat # PAB3161). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC1 polyclonal antibody (Cat # PAB3161) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 60
  • Protein Accession#:
  • NP_001092 (Gene ID : 60);P60709 (Gene ID : 60);NP_005150 (Gene ID : 70);P68032 (Gene ID : 70)
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
  • Gene Information
  • Entrez GeneID:
  • 70
  • Protein Accession#:
  • NP_001092 (Gene ID : 60);P60709 (Gene ID : 60);NP_005150 (Gene ID : 70);P68032 (Gene ID : 70)
  • Gene Name:
  • ACTC1
  • Gene Alias:
  • ACTC,CMD1R,CMH11
  • Gene Description:
  • actin, alpha, cardiac muscle 1
  • Gene Summary:
  • Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq
  • Other Designations:
  • cardiac muscle alpha actin 1
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