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Last updated: 2015/10/4
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RB1 (phospho T821) monoclonal antibody, clone 24A7

  • Catalog # : MAB6765
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of RB1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T821 of human RB1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is specific to human RB1 phosphorylated at Thr821. It can detect endogenous levels of RB1 phosphorylated at Thr821 in most cell lines.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Specificity of the RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765) as demonstrated by western blotting.
    Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765).
    Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 5925
  • Gene Name:
  • RB1
  • Gene Alias:
  • OSRC,RB,p105-Rb,pRb,pp110
  • Gene Description:
  • retinoblastoma 1
  • Gene Summary:
  • The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein
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