PTPRC monoclonal antibody, clone MEM-28 (PE)
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Specifications
Product Description
Mouse monoclonal antibody raised against native PTPRC.
Immunogen
Native purified PTPRC from human thymocytes and T lymphocytes.
Host
Mouse
Theoretical MW (kDa)
180-220
Reactivity
Human
Specificity
This antibody reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 KDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Form
Liquid
Conjugation
PE
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
Immunofluorescence (Circulating Tumor Cell) (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In stabilizing PBS, pH7.4 (0.09% sodium azide).
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Immunofluorescence (Circulating Tumor Cell)
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Gene Info — PTPRC
Entrez GeneID
5788Gene Name
PTPRC
Gene Alias
B220, CD45, CD45R, GP180, LCA, LY5, T200
Gene Description
protein tyrosine phosphatase, receptor type, C
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|glycoprotein|leukocyte-common antigen|protein tyrosine phosphatase, receptor type, c polypeptide
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Immunospecific targeting of CD45 expressing lymphoid cells: towards improved detection agents of the sentinel lymph node.
Tianqing Liu, Aidan Cousins, Chia-Chi Chien, Ivan Kempson, Sarah Thompson, Yeukuang Hwu, Benjamin Thierry.
Cancer Letters 2013 Jan; 328(2):271.
Application:Func, Mouse, Mouse lymph node, Nanoparticles, Raw 264.7 cells.
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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.
Desharnais P, Dupere-Minier G, Hamelin C, Devine P, Bernier J.
Apoptosis 2007 Dec; 13(2):197.
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Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells.
Bijian K, Zhang L, Shen SH.
Molecular Immunology 2007 May; 44(15):3682.
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Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I.
Folia Biol (Praha) 1988 Jan; 34(1):23.
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Immunospecific targeting of CD45 expressing lymphoid cells: towards improved detection agents of the sentinel lymph node.
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