APP monoclonal antibody, clone J4H9
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with APP monoclonal antibody, clone J4H9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of 293T cells. The cell was stained with APP monoclonal antibody, clone J4H9 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant APP.
Immunogen
Recombinant protein corresponding to amino acids 18-289 of human APP.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse brain tissue lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of U87 MG cells. The cell was stained with APP monoclonal antibody, clone J4H9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of 293T cells. The cell was stained with APP monoclonal antibody, clone J4H9 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — APP
Entrez GeneID
351Protein Accession#
NP_000475Gene Name
APP
Gene Alias
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2
Gene Description
amyloid beta (A4) precursor protein
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
Other Designations
A4 amyloid protein|amyloid beta A4 protein|amyloid-beta protein|beta-amyloid peptide|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II
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Interactome
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Disease
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Publication Reference
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Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal differentiation.
Ando K, Oishi M, Takeda S, Iijima K, Isohara T, Nairn AC, Kirino Y, Greengard P, Suzuki T.
Journal of Neuroscience 1999 Jun; 19(11):4421.
Application：IF, IP, WB-Ce, WB-Tr, Rat, PC-12 cells.
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Cell cycle-dependent regulation of the phosphorylation and metabolism of the Alzheimer amyloid precursor protein.
Suzuki T, Oishi M, Marshak DR, Czernik AJ, Nairn AC, Greengard P.
The EMBO Journal 1994 Mar; 13(5):1114.
Application：IP, WB-Ce, WB-Re, Human, Rat, HeLa, PC-12 cells, Synthetic peptides.
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Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal differentiation.
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