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Last updated: 2015/5/17
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JUN monoclonal antibody, clone 5B1

  • Catalog # : MAB10514
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant JUN.
  • Immunogen:
  • Recombinant protein corresponding to human JUN.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 43
  • Reactivity:
  • Human, Monkey, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) against NIH/3T3 (1) and COS-7 (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) against recombinant protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human intima canncer (A) and human rectum cancer (B) tissues using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of PC-2 cells using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with JUN monoclonal antibody, clone 5B1 (Cat # MAB10514).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells using JUN monoclonal antibody, clone 5B1 (Cat # MAB10514) (blue) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3725
  • Gene Name:
  • JUN
  • Gene Alias:
  • AP-1,AP1,c-Jun
  • Gene Description:
  • jun oncogene
  • Gene Summary:
  • This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
  • Other Designations:
  • Jun activation domain binding protein,OTTHUMP00000010036,activator protein 1,enhancer-binding protein AP1,v-jun avian sarcoma virus 17 oncogene homolog,v-jun sarcoma virus 17 oncogene homolog
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