Product Browser

Last updated: 2015/2/22
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

RB1 (phospho S807) monoclonal antibody, clone 5H12

  • Catalog # : MAB0005
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of Rb.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S807 of human Rb.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Phospho-Rb Ser807 Antibody detects endogenous Rb protein only when phosphorylated at Serine 807.
  • Recommend Usage:
  • Western Blot (1-2 ug/mL)
    Immunoprecipitation (2-4 ug/mL)
    ELISA (5-10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005). Western blot analysis of pRb in human stomach.
  • Protocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of in extracts from MOLT 4 cell using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).
  • Immunoprecipitation
  • Immunoprecipitation
  • IP-Western in HL-60 cell lysate using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).
  • ELISA
  • ELISA
  • ELISA measurement of recombinant Cdk4-CyclinD kinase activity using RB1 (phospho S807) monoclonal antibody, clone 5H12 (Cat # MAB0005).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5925
  • Gene Name:
  • RB1
  • Gene Alias:
  • OSRC,RB,p105-Rb,pRb,pp110
  • Gene Description:
  • retinoblastoma 1
  • Gene Summary:
  • The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein
  • RSS
  • YouTube
  • Linkedin
  • Facebook