mRNA Nanoparticle Carrier Mixing

mRNA exhibits therapeutic potential across considerable applications, encompassing viral vaccines, protein replacement therapies, immunotherapy, cell therapy, and genome editing. In order to elicit therapeutic effects, mRNA molecule needs to reach and enter specific target cells and generate a sufficient quantity of the desired proteins. Abnova offers mRNA mixing services for several nanoparticle carriers, including lipid nanoparticles (LNPs) and liposomes, employing either microfluidic mixing devices (Precision NanoSystems) or manual mixing methods. Microfluidic technology (Precision NanoSystems) enables the fabrication of mRNA nanoparticle carrier complex under reproducible, scalable, and controlled mixing conditions. In contrast, manual mixing presents a simpler and cost-effective alternative method.

Lipid Nanoparticles (LNPs)
Lipid nanoparticles (LNPs) consist of ionizable lipids, structural lipids, cholesterol, and PEG-grafted lipids, each playing a distinct role in the particle structure. The ionizable lipid is a key component, capable of acquiring a positive charge at a reduced pH. This allows it to bind to the negatively charged phosphate backbone of nucleic acid cargo, forming an actively enriched core protected by a lipid exterior. LNPs can be customized for various therapeutic targets, and the efficacy of the LNP hinges on these essential components.

Liposomes
Liposomes, characterized by spherical lipid bilayers surrounding an aqueous cavity, represent another rapidly advancing lipid-based nanomedicine. The selection of specific lipids and liposome properties can influence factors such as the rate of drug release, circulation time, and target organ. Hydrophobic actives can be encapsulated within the lipid bilayer's fatty chains, attached to elements on the surface, or contained within the aqueous core, offering versatility in drug delivery design.