FIS1 recombinant monoclonal antibody, clone 27B4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate with FIS1 recombinant monoclonal antibody, clone 27B4 (Cat # RAB07587).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer using FIS1 recombinant monoclonal antibody, clone 27B4 (Cat # RAB07587) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FIS1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human FIS1.
Theoretical MW (kDa)
Calculated MW: 17
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate with FIS1 recombinant monoclonal antibody, clone 27B4 (Cat # RAB07587).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer using FIS1 recombinant monoclonal antibody, clone 27B4 (Cat # RAB07587) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FIS1
Entrez GeneID
51024Protein Accession#
Q9Y3D6Gene Name
FIS1
Gene Alias
CGI-135, TTC11
Gene Description
fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Omim ID
609003Gene Ontology
HyperlinkGene Summary
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM
Other Designations
H_NH0132A01.6|tetratricopeptide repeat domain 11
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