Products

Western Blot (Cell lysate)

Western blot analysis of Hela whole cell lysate with ACO1 recombinant monoclonal antibody, clone 22C10 (Cat # RAB07573).

Immunofluorescence

Immunofluorescent staining of HepG2 Cells with ACO1 recombinant monoclonal antibody, clone 22C10 (Cat # RAB07573), counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 511-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometry shows HepG2 cells stained with ACO1 recombinant monoclonal antibody, clone 22C10 (Cat # RAB07573)(red line). The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Entrez GeneID

48

Protein Accession#

P21399

Gene Name

ACO1

Gene Alias

ACONS, IREB1, IREBP, IREBP1, IRP1

Gene Description

aconitase 1, soluble

Omim ID

100880

Gene Ontology

Hyperlink

Gene Summary

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes. [provided by RefSeq

Other Designations

OTTHUMP00000021176|OTTHUMP00000021177|OTTHUMP00000045233|aconitase 1|aconitate hydratase|citrate hydro-lyase|ferritin repressor protein|iron regulatory protein 1|iron-responsive element binding protein 1