Products

Western Blot (Cell lysate)

Western blot analysis of 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate with COMT recombinant monoclonal antibody, clone 8E1 (Cat # RAB07474).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of paraffin-embedded human brain tissue using COMT recombinant monoclonal antibody, clone 8E1 (Cat # RAB07474) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

1312

Protein Accession#

P21964

Gene Name

COMT

Gene Alias

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Gene Description

catechol-O-methyltransferase

Omim ID

116790 167870 181500

Gene Ontology

Hyperlink

Gene Summary

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq

Other Designations

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