CD27 recombinant monoclonal antibody, clone 4A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Daudi whole cell lysate, HL-60 whole cell lysate, THP-1 cell lysate, K562 cell lysate with CD27 recombinant monoclonal antibody, clone 4A1 (Cat # RAB07438).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD27.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD27.
Theoretical MW (kDa)
Calculated MW: 30
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Daudi whole cell lysate, HL-60 whole cell lysate, THP-1 cell lysate, K562 cell lysate with CD27 recombinant monoclonal antibody, clone 4A1 (Cat # RAB07438).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD27
Entrez GeneID
939Protein Accession#
P26842Gene Name
CD27
Gene Alias
MGC20393, S152, T14, TNFRSF7, Tp55
Gene Description
CD27 molecule
Omim ID
186711Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq
Other Designations
CD27 antigen|CD27L receptor|T cell activation antigen CD27|T cell antivation antigen S152|tumor necrosis factor receptor superfamily, member 7
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Interactome
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Pathway
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Disease
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