PRKCZ (phospho T560) recombinant monoclonal antibody, clone 1B10
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More Files
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Western Blot
Western blot analysis of A549 whole cell lysate with PRKCZ (phospho T560) recombinant monoclonal antibody, clone 1B10 (Cat # RAB04264).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRKCZ.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T560 of human PRKCZ.
Theoretical MW (kDa)
Calculated MW: 68 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of A549 whole cell lysate with PRKCZ (phospho T560) recombinant monoclonal antibody, clone 1B10 (Cat # RAB04264).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCZ
Entrez GeneID
5590Protein Accession#
Q05513Gene Name
PRKCZ
Gene Alias
PKC-ZETA, PKC2
Gene Description
protein kinase C, zeta
Omim ID
176982Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000001368|OTTHUMP00000044160
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Interactome
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