PTPN11 (phospho Y542) recombinant monoclonal antibody, clone 2E2
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More Files
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Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Pervanadate), Lane 2: Hela whole cell lysate (not treated), Lane 3: 293 whole cell lysate (treated with Pervanadate), Lane 4: 293 whole cell lysate (not treated), Lane 5: A549 whole cell lysate (treated with Pervanadate) and Lane 6: A549 whole cell lysate (not treated) with PTPN11 (phospho Y542) recombinant monoclonal antibody, clone 2E2 (Cat # RAB04249).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with PTPN11 (phospho Y542) recombinant monoclonal antibody, clone 2E2 (Cat # RAB04249).
Lane 1: rabbit control IgG, Lane 2: RAB04249 precipitates and Lane 3 : Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PTPN11.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y542 of human PTPN11.
Theoretical MW (kDa)
Calculated MW: 68 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (treated with Pervanadate), Lane 2: Hela whole cell lysate (not treated), Lane 3: 293 whole cell lysate (treated with Pervanadate), Lane 4: 293 whole cell lysate (not treated), Lane 5: A549 whole cell lysate (treated with Pervanadate) and Lane 6: A549 whole cell lysate (not treated) with PTPN11 (phospho Y542) recombinant monoclonal antibody, clone 2E2 (Cat # RAB04249).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with PTPN11 (phospho Y542) recombinant monoclonal antibody, clone 2E2 (Cat # RAB04249).
Lane 1: rabbit control IgG, Lane 2: RAB04249 precipitates and Lane 3 : Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTPN11
Entrez GeneID
5781Protein Accession#
Q06124Gene Name
PTPN11
Gene Alias
BPTP3, CFC, MGC14433, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2
Gene Description
protein tyrosine phosphatase, non-receptor type 11
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq
Other Designations
protein tyrosine phosphatase-2|protein-tyrosine phosphatase 2C
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Interactome
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Disease
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