FUBP1 recombinant monoclonal antibody, clone 7C3
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FUBP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human FUBP1.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Jurkat whole cell lysate; Lane 2: K562 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: Raji whole cell lysate; Lane 5: HepG2 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of FUBP1 recombinant monoclonal antibody, clone 7C3 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with FUBP1 recombinant monoclonal antibody, clone 7C3 at 1:50, counter-stained with DAPI.Immunoprecipitation
Immunoprecipitating FUBP1 in Jurkat whole cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Jurkat cells stained with FUBP1 recombinant monoclonal antibody, clone 7C3 (red line) at 1:50. -
Gene Info — FUBP1
Entrez GeneID
8880Protein Accession#
Q96AE4Gene Name
FUBP1
Gene Alias
FBP, FUBP
Gene Description
far upstream element (FUSE) binding protein 1
Omim ID
603444Gene Ontology
HyperlinkGene Summary
This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq
Other Designations
DNA helicase V|FUSE-binding protein|OTTHUMP00000038483|far upstream element binding protein|far upstream element-binding protein
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