AURKB recombinant monoclonal antibody, clone 4E5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: 293 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: U251 whole cell lysate; Lane 6: A549 whole cell lysate; Lane 7: HepG2 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of AURKB recombinant monoclonal antibody, clone 4E5 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system.
Immunoprecipitation
Immunoprecipitating AURKB in Hela whole cell lysate.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AURKB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human AURKB.
Theoretical MW (kDa)
Calculated MW: 40, 3
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: 293 whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: U251 whole cell lysate; Lane 6: A549 whole cell lysate; Lane 7: HepG2 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of AURKB recombinant monoclonal antibody, clone 4E5 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system.Immunoprecipitation
Immunoprecipitating AURKB in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AURKB
Entrez GeneID
9212Protein Accession#
Q96GD4Gene Name
AURKB
Gene Alias
AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, STK12, STK5
Gene Description
aurora kinase B
Omim ID
604970Gene Ontology
HyperlinkGene Summary
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM
Other Designations
aurora-1|aurora-B|serine/threonine kinase 12
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Interactome
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Disease
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