UBE2C recombinant monoclonal antibody, clone SAIC-41A-1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide corresponding to peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
Sequence
GISAFPESDNLFK
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow cytometry
Immunofluorescence
Immuno-MRM
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Caco-2, MCF7, HeLa cell lysate using anti-UBE2C recombinant monoclonal antibody, clone SAIC-41A-1 (Cat # RAB03921).Immunofluorescence
Immunofluorescence analysis of UBE2C (green) in Caco-2 cells using human anti-UBE2C recombinant monoclonal antibody, clone SAIC-41A-1 (Cat # RAB03921), and DAPI(blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow-cytometry analysis of UBE2C in MCF7 cells using human anti-UBE2C recombinant monoclonal antibody, clone SAIC-41A-1 (Cat # RAB03921).Immuno-MRM (multiple reaction monitoring)
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Gene Info — UBE2C
Entrez GeneID
11065Protein Accession#
O00762Gene Name
UBE2C
Gene Alias
UBCH10, dJ447F3.2
Gene Description
ubiquitin-conjugating enzyme E2C
Omim ID
605574Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000031653|OTTHUMP00000031655|cyclin-selective ubiquitin carrier protein|mitotic-specific ubiquitin-conjugating enzyme|ubiquitin carrier protein E2-C|ubiquitin-protein ligase C
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