S100B recombinant monoclonal antibody, clone S100B/1706R
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against full length human recombinant S100B protein.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human recombinant S100B protein
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Concentration
0.2 mg/mL
Isotype
IgG
Recommend Usage
Flow cytometry (0.5-1 ug/million cells in 0.1mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(0.25-0.5 ug/mL for 30 min at RT)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage Instruction
Store at 2~8°C.
Aliquot to avoid repeated freezing and thawing.Note
Optimal dilutions for each application to be determined by the researcher
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of human melanoma with anti-S100B recombinant monoclonal antibody, clone S100B/1706R (Cat # RAB03831).Immunofluorescence
Flow Cytometry
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Gene Info — S100B
Entrez GeneID
6285Protein Accession#
P04271Gene Name
S100B
Gene Alias
NEF, S100, S100beta
Gene Description
S100 calcium binding protein B
Omim ID
176990Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq
Other Designations
OTTHUMP00000174958|S-100 calcium-binding protein, beta chain|S100 beta|S100 calcium binding protein, beta (neural)|S100 calcium-binding protein, beta|S100 calcium-binding protein, beta (neural)
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Interactome
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Disease
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