Il6ra recombinant monoclonal antibody, clone D7715A7
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Il6ra recombinant monoclonal antibody, clone D7715A7 (Cat # RAB03591).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ coverslips and stained with the chimeric r version of RAB03591 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03591
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Il6ra recombinant monoclonal antibody, clone D7715A7 (Cat # RAB03591).
Paraformaldehyde fixed mouse splenocytes were stained with anti-Fluorescein IgG antibody (isotype control-shaded line) or the r version of RAB03591 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Il6ra.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against OKT4 cells by immunising Lewis rats.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Il6ra recombinant monoclonal antibody, clone D7715A7 (Cat # RAB03591).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ coverslips and stained with the chimeric r version of RAB03591 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03591
(B) DAPI
(C) Merged channels
(D) Isotype controlBlocking
Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Il6ra recombinant monoclonal antibody, clone D7715A7 (Cat # RAB03591).
Paraformaldehyde fixed mouse splenocytes were stained with anti-Fluorescein IgG antibody (isotype control-shaded line) or the r version of RAB03591 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Il6ra
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