Il10ra recombinant monoclonal antibody, clone 1B1.3a
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More Files
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Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Il10ra recombinant monoclonal antibody, clone 1B1.3a (Cat # RAB03561).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03561 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03561
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of RAW264.7 cells with Il10ra recombinant monoclonal antibody, clone 1B1.3a (Cat # RAB03561).
Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03561 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Il10ra.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against the purified recombinant extracellular region of mouse Il10ra.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
Flow Cytometry
IImmunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Il10ra recombinant monoclonal antibody, clone 1B1.3a (Cat # RAB03561).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03561 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03561
(B) DAPI
(C) Merged channels
(D) Isotype controlBlocking
Flow Cytometry
Flow cytometric analysis of RAW264.7 cells with Il10ra recombinant monoclonal antibody, clone 1B1.3a (Cat # RAB03561).
Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03561 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Il10ra
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