GSTP1 recombinant monoclonal antibody, clone SAIC-22D-22
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More Files
- More Functions
Immunofluorescence
Immunofluorescenct staining of Hela cells with GSTP1 recombinant monoclonal antibody, clone SAIC-22D-22 (Cat # RAB03500).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03500 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1500, showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03500
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of Hela cells with GSTP1 recombinant monoclonal antibody, clone SAIC-22D-22 (Cat # RAB03500).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03500 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GSTP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against peptide "YISLIYTNYEAGK" derived from Glutathione S-transferase P conjugated to KLH.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Immuno-MRM (multiple reaction monitoring)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescenct staining of Hela cells with GSTP1 recombinant monoclonal antibody, clone SAIC-22D-22 (Cat # RAB03500).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03500 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1500, showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03500
(B) DAPI
(C) Merged channels
(D) Isotype controlFlow Cytometry
Flow cytometric analysis of Hela cells with GSTP1 recombinant monoclonal antibody, clone SAIC-22D-22 (Cat # RAB03500).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03500 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Immuno-MRM (multiple reaction monitoring)
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Gene Info — GSTP1
Entrez GeneID
2950Gene Name
GSTP1
Gene Alias
DFN7, FAEES3, GST3, PI
Gene Description
glutathione S-transferase pi 1
Omim ID
134660Gene Ontology
HyperlinkGene Summary
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq
Other Designations
OTTHUMP00000174659|deafness, X-linked 7|fatty acid ethyl ester synthase III|glutathione transferase
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Interactome
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