GCN4 recombinant monoclonal antibody, clone C11L34
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against yeast GCN4.
Antibody Species
Mouse
Immunogen
Original antibody is raised against biotinylated GCN4-peptide (HLENEVARLKK).
Reactivity
Yeast
Form
Liquid
Isotype
IgG1 lambda
Recommend Usage
Immunofluorescence
Immunoprecipitation
Surface Plasmon Resonance
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of Hela cells expressing mCherry tagged GCN4 with GCN4 recombinant monoclonal antibody, clone C11L34 (Cat # RAB03482).
Immunofluorescence analysis of fixed Hela cells stained with the chimeric mouse IgG1 version of RAB03482 followed by Alexa Fluor® 488 secondary antibody, showing mitochondrial staining. The nuclear and mitochondiral DNA stain is DAPI (blue). Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody.Immunoprecipitation
Surface Plasmon Resonance
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Gene Info — GCN4
Entrez GeneID
856709Gene Name
GCN4
Gene Alias
AAS3, ARG9
Gene Description
Gcn4p
Gene Ontology
HyperlinkOther Designations
Transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
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