MYC tag recombinant monoclonal antibody, clone 9E10
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HEK293 spiked with 5 ng multitag protein (35 ug protein in RIPA buffer), Lane 2: HEK293 unspiked with 5 ng multitag protein (35 ug protein in RIPA buffer) and Lane 3: positive control contains 25 ng purified multitag protein with MYC tag recombinant monoclonal antibody, clone 9E10 (Cat # RAB03380) (2 ug/mL). Primary incubation was 1 hour. Detected by chemiluminescence.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MYC tag recombinant monoclonal antibody, clone 9E10 (Cat # RAB03380).
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human MYC.
Antibody Species
Mouse
Immunogen
Original antibody is raised against a synthetic peptide corresponding to amino acids 408-439 from C-terminus of human MYC.
Reactivity
Human
Form
Liquid
Isotype
IgG1 kappa
Recommend Usage
ELISA
Immunofluorescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: HEK293 spiked with 5 ng multitag protein (35 ug protein in RIPA buffer), Lane 2: HEK293 unspiked with 5 ng multitag protein (35 ug protein in RIPA buffer) and Lane 3: positive control contains 25 ng purified multitag protein with MYC tag recombinant monoclonal antibody, clone 9E10 (Cat # RAB03380) (2 ug/mL). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of MYC tag recombinant monoclonal antibody, clone 9E10 (Cat # RAB03380). -
Gene Info — MYC
Entrez GeneID
4609Gene Name
MYC
Gene Alias
bHLHe39, c-Myc
Gene Description
v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
Other Designations
avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|v-myc avian myelocytomatosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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