FCER2 recombinant monoclonal antibody, clone CAT-13.9C1
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of Daudi cells with FCER2 recombinant monoclonal antibody, clone CAT-13.9C1 (Cat # RAB03198).
Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version RAB03198 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03198- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Daudi cells with FCER2 recombinant monoclonal antibody, clone CAT-13.9C1 (Cat # RAB03198).
Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03198 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FCER2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens.
Sequence
This antibody was raised by immunising mice with with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of Daudi cells with FCER2 recombinant monoclonal antibody, clone CAT-13.9C1 (Cat # RAB03198).
Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version RAB03198 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03198- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of Daudi cells with FCER2 recombinant monoclonal antibody, clone CAT-13.9C1 (Cat # RAB03198).
Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03198 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — FCER2
Entrez GeneID
2208Gene Name
FCER2
Gene Alias
CD23, CD23A, CLEC4J, FCE2, IGEBF
Gene Description
Fc fragment of IgE, low affinity II, receptor for (CD23)
Omim ID
151445Gene Ontology
HyperlinkGene Summary
The human leukocyte differentiation antigen CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.[supplied by OMIM
Other Designations
C-type lectin domain family 4, member J|CD23 antigen|Fc fragment of IgE, low affinity II, receptor for (CD23A)
-
Interactome
-
Pathway
-
Disease
- Asthma
- Birth Weight
- Bronchiolitis
- Genetic Predisposition to Disease
- Glioblastoma
- Glioma
- Infant
- Leukemia
- Lung Neoplasms
+ View More Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com