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Immunohistochemistry (Frozen sections)

Immunofluorescence

Flow Cytometry

Flow cytometric analysis of murine neutrophils with ITGAM recombinant monoclonal antibody, clone 5C6 (Cat # RAB03157).
Ex vivo murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with RAB03157 and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Figure shows staining first with RAB03157 or isotype control (.1) followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control- cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using RAB03157 compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive- agreeing with findings obtained using hybridoma-derived antibody.

Entrez GeneID

3684

Gene Name

ITGAM

Gene Alias

CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6

Gene Description

integrin, alpha M (complement component 3 receptor 3 subunit)

Omim ID

120980

Gene Ontology

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Gene Summary

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

antigen CD11b (p170)|complement component receptor 3, alpha|integrin alpha M|macrophage antigen alpha polypeptide|neutrophil adherence receptor alpha-M subunit