CXCR5 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis with CXCR5 recombinant monoclonal antibody (Cat # RAB02646) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded mouse kidney using CXCR5 recombinant monoclonal antibody (Cat # RAB02646). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CXCR5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant human CXCR5.
Theoretical MW (kDa)
42
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of CXCR5 protein.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.01M TBS, pH7.4 (1% BSA, 0.03% Proclin300 and 50% Glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles. -
Applications
Western Blot
Western blot analysis with CXCR5 recombinant monoclonal antibody (Cat # RAB02646) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded mouse kidney using CXCR5 recombinant monoclonal antibody (Cat # RAB02646). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C. -
Gene Info — CXCR5
Entrez GeneID
643Protein Accession#
P32302Gene Name
CXCR5
Gene Alias
BLR1, CD185, MDR15, MGC117347
Gene Description
chemokine (C-X-C motif) receptor 5
Omim ID
601613Gene Ontology
HyperlinkGene Summary
This gene was identified as a gene specifically expressed in Burkitt's lymphoma and lymphatic tissues. The protein encoded by this gene is predicted to be a seven transmembrane G protein- coupled receptor and belongs to the CXC chemokine receptor family. BLC, a B-lymphocyte chemoattractant, was identified to be a specific ligand for this receptor. Studies of this gene and its mouse conterpart strongly suggest the essential function of this gene in B cell migration and localization within specific anatomic compartments, such as follicles in lymph nodes as well as in spleen. Two alternatively spliced variants of this gene exist. [provided by RefSeq
Other Designations
Burkitt lymphoma receptor 1|Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5)|Burkitt lymphoma receptor 1, GTP-binding protein|C-X-C chemokine receptor type 5|monocyte-derived receptor 15
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Interactome
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Pathway
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Disease
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