HSF1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Hela (A), Jurkat (B) whole cell lysates with HSF1 recombinant monoclonal antibody (Cat # RAB02509).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using HSF1 recombinant monoclonal antibody (Cat # RAB02509). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.14). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSF1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human HSF1.
Theoretical MW (kDa)
80
Reactivity
Human
Specificity
Recognizes endogenous levels of HSF1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A), Jurkat (B) whole cell lysates with HSF1 recombinant monoclonal antibody (Cat # RAB02509).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using HSF1 recombinant monoclonal antibody (Cat # RAB02509). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.14). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — HSF1
Entrez GeneID
3297Protein Accession#
Q00613Gene Name
HSF1
Gene Alias
HSTF1
Gene Description
heat shock transcription factor 1
Omim ID
140580Gene Ontology
HyperlinkGene Summary
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq
Other Designations
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