ACOX1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Raw264.7 (A), mouse liver (B) whole cell lysates with ACOX1 recombinant monoclonal antibody (Cat # RAB02463).
Immunofluorescence
Immunofluorescent analysis of HeLa cells with ACOX1 recombinant monoclonal antibody (Cat # RAB02463). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse ACOX1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of mouse ACOX1.
Theoretical MW (kDa)
50, 72
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of ACOX1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Raw264.7 (A), mouse liver (B) whole cell lysates with ACOX1 recombinant monoclonal antibody (Cat # RAB02463).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with ACOX1 recombinant monoclonal antibody (Cat # RAB02463). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — ACOX1
Entrez GeneID
51Protein Accession#
Q15067Gene Name
ACOX1
Gene Alias
ACOX, MGC1198, PALMCOX, SCOX
Gene Description
acyl-Coenzyme A oxidase 1, palmitoyl
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
acyl-CoA oxidase, straight-chain|peroxisomal fatty acyl-CoA oxidase
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Interactome
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Pathway
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