HMGA1 recombinant monoclonal antibody, clone R01-9D3
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela, Lane 2: C6 and Lane 3: MCF-7 lysates with HMGA1 recombinant monoclonal antibody, clone R01-9D3 (Cat # RAB02215).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with HMGA1 recombinant monoclonal antibody, clone R01-9D3 (Cat # RAB02215). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HMGA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HMGA1.
Theoretical MW (kDa)
Calculated MW: 12 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela, Lane 2: C6 and Lane 3: MCF-7 lysates with HMGA1 recombinant monoclonal antibody, clone R01-9D3 (Cat # RAB02215).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with HMGA1 recombinant monoclonal antibody, clone R01-9D3 (Cat # RAB02215). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — HMGA1
Entrez GeneID
3159Protein Accession#
P17096Gene Name
HMGA1
Gene Alias
HMG-R, HMGA1A, HMGIY, MGC12816, MGC4242, MGC4854
Gene Description
high mobility group AT-hook 1
Omim ID
600701Gene Ontology
HyperlinkGene Summary
This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016222|OTTHUMP00000016223|OTTHUMP00000016224|OTTHUMP00000039618|high-mobility group (nonhistone chromosomal) protein isoforms I and Y|nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y
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Interactome
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Disease
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