HNRNPM recombinant monoclonal antibody, clone R06-3B1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HNRNPM.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HNRNPM.
Theoretical MW (kDa)
Calculated MW: 78 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6, Lane 3: 3T3 and Lane 4: Hela lysates with HNRNPM recombinant monoclonal antibody, clone R06-3B1 (Cat # RAB02209).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with HNRNPM recombinant monoclonal antibody, clone R06-3B1 (Cat # RAB02209). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry
Immunofluorescence
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Gene Info — HNRNPM
Entrez GeneID
4670Protein Accession#
P52272Gene Name
HNRNPM
Gene Alias
DKFZp547H118, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1
Gene Description
heterogeneous nuclear ribonucleoprotein M
Omim ID
160994Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Multiple alternatively spliced transcript variants are known for this gene but only two transcripts has been isolated. [provided by RefSeq
Other Designations
M4 protein|N-acetylglucosamine receptor 1|heterogenous nuclear ribonucleoprotein M|heterogenous nuclear ribonucleoprotein M4|hnRNA-binding protein M4
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