IRAK4 recombinant monoclonal antibody, clone R01-1B2
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Jurkat and Lane 2: Hela lysates with IRAK4 recombinant monoclonal antibody, clone R01-1B2 (Cat # RAB02163).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with IRAK4 recombinant monoclonal antibody, clone R01-1B2 (Cat # RAB02163). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse IRAK4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human IRAK4.
Theoretical MW (kDa)
Calculated MW: 52 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Jurkat and Lane 2: Hela lysates with IRAK4 recombinant monoclonal antibody, clone R01-1B2 (Cat # RAB02163).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with IRAK4 recombinant monoclonal antibody, clone R01-1B2 (Cat # RAB02163). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunofluorescence
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Gene Info — IRAK4
Entrez GeneID
51135Protein Accession#
Q9NWZ3Gene Name
IRAK4
Gene Alias
IPD1, NY-REN-64, REN64
Gene Description
interleukin-1 receptor-associated kinase 4
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
Other Designations
IRAK-4 mutated form 1|interleukin-1 receptor associated kinase 4
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Interactome
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Pathway
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Disease
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