PFKP recombinant monoclonal antibody, clone R05-4H9
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PFKP.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human PFKP.
Theoretical MW (kDa)
Calculated MW: 86 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: C6, Lane 2: 3T3 and Lane 3: Hela lysates with PFKP recombinant monoclonal antibody, clone R05-4H9 (Cat # RAB02007).Immunocytochemistry
Immunocytochemical staining of Hela with PFKP recombinant monoclonal antibody, clone R05-4H9 (Cat # RAB02007). (A) PFKP (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PFKP
Entrez GeneID
5214Protein Accession#
Q01813Gene Name
PFKP
Gene Alias
FLJ40226, PFK-C, PFKF
Gene Description
phosphofructokinase, platelet
Omim ID
171840Gene Ontology
HyperlinkGene Summary
The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM
Other Designations
OTTHUMP00000018966|Phosphofructokinase, platelet type
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
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