PFKM recombinant monoclonal antibody, clone R08-6B4
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More Files
- More Functions
Western Blot
Western blot analysis of Fructose 6 Phosphate Kinase in C6, 3T3, Hela lysates using human Fructose 6 Phosphate Kinase recombinant monoclonal antibody, clone R08-6B4 (Cat # RAB01718).
Immunocytochemistry
Immunocytochemical staining of Hela with Fructose 6 Phosphate Kinase recombinant monoclonal antibody, clone R08-6B4 (Cat # RAB01718). (A) Fructose 6 Phosphate Kinase (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Fructose 6 Phosphate Kinase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Fructose 6 Phosphate Kinase
Theoretical MW (kDa)
Calculated MW: 85 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Fructose 6 Phosphate Kinase in C6, 3T3, Hela lysates using human Fructose 6 Phosphate Kinase recombinant monoclonal antibody, clone R08-6B4 (Cat # RAB01718).Immunocytochemistry
Immunocytochemical staining of Hela with Fructose 6 Phosphate Kinase recombinant monoclonal antibody, clone R08-6B4 (Cat # RAB01718). (A) Fructose 6 Phosphate Kinase (green) and (B) DAPI (blue). -
Gene Info — PFKM
Entrez GeneID
5213Protein Accession#
P08237Gene Name
PFKM
Gene Alias
GSD7, MGC8699, PFK-1, PFK-M, PFKX
Gene Description
phosphofructokinase, muscle
Gene Ontology
HyperlinkGene Summary
The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM
Other Designations
phosphofructokinase, muscle type|phosphofructokinase, polypeptide X
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Fructose and mannose metabolism
- Galactose metabolism
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