FOXO1 recombinant monoclonal antibody, clone R07-7C4
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human FOXO1A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human FOXO1A
Theoretical MW (kDa)
Calculated MW: 70 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of FOXO1A in HepG2 lysates using human FOXO1A recombinant monoclonal antibody, clone R07-7C4 (Cat # RAB01659).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with FOXO1A recombinant monoclonal antibody, clone R07-7C4 (Cat # RAB01659). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical staining of Hela with FOXO1A recombinant monoclonal antibody, clone R07-7C4 (Cat # RAB01659). (A) FOXO1A (green) and (B) DAPI (blue). -
Gene Info — FOXO1
Entrez GeneID
2308Protein Accession#
Q12778Gene Name
FOXO1
Gene Alias
FKH1, FKHR, FOXO1A
Gene Description
forkhead box O1
Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018301|forkhead box O1A (rhabdomyosarcoma)|forkhead homolog in rhabdomyosarcoma|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
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Interactome
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Pathway
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Disease
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