AGO2 recombinant monoclonal antibody, clone R04-5H0
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More Files
- More Functions
Western Blot
Western blot analysis of Argonaute 2 in K562, MCF-7, Hela lysates using human Argonaute-2 recombinant monoclonal antibody, clone R04-5H0 (Cat # RAB01592).
Immunocytochemistry
Immunocytochemistry analysis of Argonaute 2 (green) in HCT116 using human Argonaute-2 recombinant monoclonal antibody, clone R04-5H0 (Cat # RAB01592), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Argonaute-2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Argonaute-2
Theoretical MW (kDa)
Calculated MW: 97 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Argonaute 2 in K562, MCF-7, Hela lysates using human Argonaute-2 recombinant monoclonal antibody, clone R04-5H0 (Cat # RAB01592).Immunocytochemistry
Immunocytochemistry analysis of Argonaute 2 (green) in HCT116 using human Argonaute-2 recombinant monoclonal antibody, clone R04-5H0 (Cat # RAB01592), and DAPI(blue).Immunoprecipitation
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Gene Info — EIF2C2
Entrez GeneID
27161Protein Accession#
Q9UKV8Gene Name
EIF2C2
Gene Alias
AGO2, MGC3183, Q10
Gene Description
eukaryotic translation initiation factor 2C, 2
Omim ID
606229Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
argonaute 2
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Interactome
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Disease
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